Dyno Therapeutics

Job Title: Sr. Scientist – AAV Engineering, Technology Development

Job Number: 24973

Location: Cambridge, MA

Job Description

In coordination with scientific leadership, you would play a central role in designing and implementing new technologies that are essential for Dyno’s pipeline. These technologies may be in close coordination with one of our on-going projects or related to streamlining our platform. Successfully meeting these challenges will require not only a broad range of molecular biology skills, independent thinking, and an aptitude for troubleshooting, but also a strong, creative drive to develop new assays as the need arises. Your work in this role would be in collaboration with scientists and programmers throughout Dyno, learning and contributing to many aspects of our pipeline, including the iterative design and production of next-gen AAV libraries. You would be responsible for understanding project goals and for creatively optimizing solutions to ensure their success.

Responsibilities

  • Design methods and technologies that may directly support a project or streamline our platform
  • Technology development examples may include but are not limited to:
  • Combinatorial library cloning to produce and screen enhanced synthetic AAV capsids in high throughput
  • Medium throughput assays to validate performance of synthetic capsids in vivo
  • RNA/DNA target-sequence enrichment strategies for in vivo transduction measurement
  • New and improved ITR and transgene expression constructs
  • Implementing such methods may require but are not limited to:
  •  NGS sample preparation procedures
  • Advanced cloning at very high efficiencies
  • Work with cultured cells, as well as tissues
  • Share recommendations and insight for pipeline improvement toward higher throughput and efficiency
  • Collaborate with a diverse team of wet-lab scientists, protein engineers, and computational scientists
  • Assist in lab organization, including ordering supplies and restocking

Required Skills

  • PhD in biology, biochemistry, biomedical engineering, or a related field
  • 5+ years hands-on wet lab experience
  • Established experience in advanced cloning techniques (Type IIs, Gateway, Gibson, etc.)
  • Expertise in DNA/RNA analysis and titering protocols (qPCR, RT-qPCR, etc.)
  • Knowledgeable in Next Gen Sequencing and library preparation
  • Skilled in sequence design and analysis software, e.g., BLAST, Geneious, Benchling, etc. 
  • Strong collaborative, organizational, and presentation skills
  • Ability to document, maintain, and share an organized account of lab work and results

Preferred qualifications

  • 2+ years post PhD experience
  • Background in AAV research (or additional viruses)
  • Experience in mammalian cell culture (Transfection, transduction)
  • Experience with cell and tissue analysis – staining, FACS, cell-sorting, imaging, histology, microscopy, etc.
  • Experience in single cell RNA sequencing
  • Experience in animal studies
  • Skilled in protein analysis methods – SDS-PAGE, Bradford, ELISA, Western blots (FLAG, HIS, HA) 
  • Experience with digital droplet PCR

 

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