1-617-500-6690 [email protected]

Exo Therapeutics

Job Title: Senior/Principal Research Associate, Biology

Job Number: 30599

Location: Watertown, MA

Job Description

Exo Therapeutics seeks a highly motivated biologist with a strong background in cell-based assay development. This position will be an integral part of the biology group and will help drive the drug discovery programs forward. You will work across therapeutic functions with a primary focus in oncology to help discover and develop innovative therapeutics.

This position is predominantly laboratory/wet bench based and requires strong experimental technique, the ability to deliver quality data in a timely manner from high throughput cell-based assays and work effectively with team members in support of small molecule drug discovery efforts. Data analysis, interpretation and assay development experience is required.

Responsibilities

  • Design and execute experiments, and perform data analysis
  • Design, QC, validate, and execute medium- to high- throughput 96/384 well assay formats, such as ELISA, quantitative Western, high content IF cell imaging, cell viability/death utilizing small molecules to generate full dose response curves
  • Leverage multiple assay platforms to measure target engagement, pathway modulation and cell sensitivity in response to compounds or target modulation
  • Perform molecular techniques including DNA/RNA isolation, cloning, PCR, qRT-PCR, gene expression analysis, cell line engineering as needed
  • Identify and characterize appropriate cell models that are dependent on the target of interest
  • Utilize compounds and molecular target manipulation (e.g. RNAi) to dissect target function
  • Interact and collaborate with biology and chemistry team members to contribute to drug discovery and development program progression, present data at regular team meetings
  • Document, compile and interpret experimental data; manage laboratory notebook, databases, and/or spreadsheets to present data in a meaningful manner

Required Skills

  • BS/MS with at least 5+ years of experience in in Vitro Pharmacology, Cell biology or a related discipline degree with significant proven experience, preferably in the Pharmaceutical or Biotechnology industry
  • Prior experience working and managing CRO partnerships preferred

Experienced in:

  • Aseptic tissue culture techniques and mammalian cell tissue culture
  • Protein lysate preparation from cells and tissues, and quantitative Western blotting
  • Molecular techniques including DNA/RNA isolation, cloning, and PCR/ qRT-PCR
  • Cellular functional assays such as cytotoxicity, apoptosis, and siRNA/shRNA transfection
  • Working with small molecules to generate full dose response curves in drug discovery
  • Development and optimization of high throughput (96/384 well) cell-based assays using a variety of readout technologies (e.g. fluorescence, luminescence, AlphaLISA)
  • Various bioassays such as High content IF imaging, ELISA, multiplex protein analysis is preferred
  • Ability to work as a team player and be able to work on several projects simultaneously
  • Understanding of oncology and/or immunology is desirable

All fully qualified applicants who are authorized to work in the US at the time of application will receive consideration for employment without regard to race, religion, gender, sexual orientation, national origin, ancestry, disability, veteran status, as protected under law.

 

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