Research Technician II – WashU Neurosurgery & Genetics

Job Title: Research Technician II – WashU Neurosurgery & Genetics

Job Number: 61916

Location: St. Louis, US

Job Description

Position Summary

Position available in the laboratory of Yang E. Li in the Department of Neurological Surgery and Genetics, Washington University School of Medicine (WUSM).

Epigenomics is at the forefront of Precision Medicine. The epigenome plays a central role in regulating the functional output of the genome during development. Changes in the epigenome that result from exposure to stress, pathogenic conditions and during aging can determine the patterns of gene expression and predispose an individual to diseases. Epigenome research promises to significantly improve our understanding of the factors that drive human development and disease. Our laboratory aims to develop innovative computational tools/methods and utilize most advanced single-cell (epi)genomic techniques to gain a comprehensive understanding of gene regulation in mammalian models and human diseases, particularly in brain tumors and neuropsychiatric disorders.

The Research Technician will be trained to perform experiments to map cells’ epigenomes such as ATAC-seq, single-cell ATAC-seq, single-cell RNA-seq, and single-cell multiomics assays such as single-cell ATAC- + RNA-seq, single-cell Methyl-HiC, and and Paired-Tag. The technician will assist in producing high-quality data for multiple research efforts across departments. They will be responsible for careful record keeping, operating laboratory equipment, troubleshooting, quality control, and periodically summarizing results for supervisors. Additional duties may include organization of laboratory reagents, and maintenance of laboratory equipment. We seek a candidate who is enthusiastic about learning cutting-edge genomic techniques and working in a dynamic “start-up” atmosphere similar to what might be found in the biotechnology sector, yet within a vibrant academic research university.

Assists with the technical aspects of studies and experiments, including documentation and preparation of materials. 

Job Description

For information on the lab please visit https://yelilab.wustl.edu

Primary Duties & Responsibilities

• Assists with research studies, experiments and assays, including collection of data, preparation of solutions and set-up and maintenance of equipment.
• Performs data entry and maintains data files on research.
• Prepares tissue cultures, reagents, probe tests, etc.
• Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions.
• Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures.
• Assists with general lab maintenance and cleaning.
• Performs other duties as assigned.

Working Conditions

This position works in a laboratory environment with potential exposure to biological and chemical hazards. The individual must be physically able to wear protective equipment and to provide standard care to research animals.

Preferred Qualifications

• Experience with the operation of a personal computer and software application, such as MS Word and Excel.
• Experience in a UNIX command environment is a big plus.
• Hands-on experience with epigenomic assays such as ChIP-seq, RNA-seq, or ATAC-seq.
• Hands-on experience with high end laboratory equipment such as robotic platforms, cell sorters, or DNA sequencers.
• Strong organizational skills and attention to detail.
• Experience with general lab maintenance including cleaning, stocking supplies.
• Ability to perform molecular biology techniques such as PCR, DNA preparation/extraction, and gel electrophoresis.
• Demonstrated ability to work independently and communicate with laboratory staff and investigators.
• Ability to maintain complete and organized records/reports.
• Working knowledge of computer software and general lab equipment.

Application Deadline: 2023-12-31