Dyno Therapeutics

Job Title: Research Associate – AAV Engineering, Virus Production

Job Number: 24975

Location: Cambridge, MA

Job Description

Under the guidance of senior researchers, you would support a number of stages in the Dyno pipeline, including the production and analysis of complex engineered AAV vector libraries. Consistency, clever protocol optimization, and precision are key to Dyno’s workflow. This important role contributes to our company’s vector engineering and evaluation processes and calls for an established background in cell culture, a Molecular Biology skillset, and the ability to learn, develop, and optimize detailed protocols as the need arises. You would collaborate with scientists and computational biologists throughout Dyno – learning from and contributing to a diverse set of projects – and you would be responsible for understanding project goals and for creatively developing solutions to ensure their success.

Responsibilities

  • Maintain cultured cell lines
  • Assist in AAV production and quantification
  • Perform transfections and transductions to evaluate novel virus designs
  • Assist in lab organization, including ordering supplies and restocking 
  • Follow lab protocols and safety rules

Required Skills

  • BA or MA in biology, biochemistry, biomedical engineering, or related fields
  • 2+ years hands-on wet lab experience
  • Highly skilled at cell culture: 
    • maintenance 
    • transfection 
    • microscopy/imaging
  • Experienced in standard Molecular biology techniques, such as PCR, gel electrophoresis, imaging, and cloning
  • Ability to conduct advanced protocols and procedures precisely and consistently
  • Ability to document and maintain an organized account of lab work and results

Preferred qualifications:

  • Experienced in virus (preferably AAV) production, purification, titration, and transduction
  • Highly Skilled in the following Molecular biology methods:
    • Standard and Gibson Cloning: bacterial transformation, culture, plating, and plasmid purification
    • qPCR
    • Western blot
    • Elisa
    • FACs
  • Knowledge of oligo/primer/probe/gBlock design strategies
  • Sequence design and analysis software skills, such as Benchling and BLAST

 

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