Dyno Therapeutics

Job Title: Research Associate – AAV Engineering, Tissue Analysis

Job Number: 24977

Location: Cambridge, MA

Job Description

Under the guidance of senior research staff, you would be involved in the extraction and purification of nucleic acids and proteins from mammalian cells and tissues, followed by detailed analysis prior to high-throughput sequencing. This role requires a broad Molecular Biology skillset and the ability to learn, develop, and optimize in vitro assays as the need arises. Your work in this role would be in collaboration with scientists and programmers throughout Dyno. Therefore, you would learn from and contribute to a diverse set of projects. You would be responsible for understanding project goals and for creatively optimizing solutions to ensure their success.

Responsibilities

  • Assist in sample tracking and organization of mammalian cells and tissues
  • Extract, purify, and enrich nucleic acid and protein from mammalian cells and tissues 
  • Follow and optimize ex vivo nucleic acid quantification assays (e.g., qPCR, RT-qPCR)
  • Perform and troubleshoot protein analysis methods (e.g., Bradford, Western Blots, ELISA)
  • Implement next-gen sequencing DNA preparation procedures (PCR amplification, gel extraction)
  • Assist in lab organization, including ordering supplies and restocking 
  • Follow lab protocols and safety regulations

Required Skills

  • BA or MA in biology, biochemistry, biomedical engineering, or related fields
  • 2+ years hands-on wet lab experience
  • Experience in tissue handling 
  • Experience in DNA/RNA purification and analysis protocols (qPCR, RT-qPCR, etc.)
  • Skilled in protein extraction and analysis methods – Bradford, ELISA, Western blots 
  • Molecular biology proficiency: PCR, agarose gel electrophoresis and imaging, gel excision and purification 
  • Ability to follow advanced protocols and procedures
  • Ability to document and maintain an organized account of lab work and results
  • Skilled in Excel/Prism, Word, PowerPoint, and other relevant software 

Preferred qualifications

  • Focused experience in tissue processing, RNA/DNA quantification (qPCR), and quality control
  • Experience in histology
  • Cloning skills
  • Cell culture experience
  • Cell analysis – microscopy, staining, FACS, cell-sorting
  • Skilled in sequence design and analysis software, e.g., BLAST, Geneious, Benchling, etc. 

 

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