Dyno Therapeutics

Job Title: Research Associate – AAV Engineering, Library Assembly & NGS

Job Number: 24976

Location: Cambridge, MA

Job Description

Under the guidance of senior researchers, you would support a number of stages in the Dyno pipeline, including the assembly of complex AAV vector libraries from synthesized DNA and the preparation of AAV library samples for Next-Gen Sequencing analysis. Consistency, clever protocol optimization, and precision are key to Dyno’s workflow. This important role caps our vector engineering process on both ends, and calls for an established Molecular Biology skillset and the ability to learn, develop, and optimize detailed protocols as the need arises. You would collaborate with scientists and computational biologists throughout Dyno – learning from and contributing to a diverse set of projects – and you would be responsible for understanding project goals and for creatively developing solutions to ensure their success.  

Responsibilities

  • Clone complex library assemblies for AAV production 
  • Execute large scale DNA preparation protocols for transfection 
  • Implement next-gen sequencing DNA preparation procedures (amplification/ligation) 
  • Assist in lab organization, including ordering supplies and restocking 
  • Follow lab protocols and safety rules 

Required Skills

  • BA or MA in biology, biochemistry, biomedical engineering, or related fields 
  • 2+ years hands-on wet lab experience
  • Highly skilled in the following Molecular biology skills:
    • PCR
    • Agarose gel electrophoresis and imaging 
    • Cloning: bacterial transformation, culture, plating, and plasmid purification 
  • Knowledge of oligo/primer/probe/gBlock design strategies 
  • Ability to conduct advanced protocols and procedures precisely and consistently 
  • Ability to document and maintain an organized account of lab work and results 

Preferred qualifications:

  • Focused experience in advanced DNA cloning methodologies 
  • Focused experience in RNA/DNA quantification (qPCR) and quality control 
  • Cell culture skills: maintenance, transfection, virus titration, and virus transduction 
  • Sequence design and analysis software skills, such as Benchling and BLAST 

 

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